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According to Fujita , during the initial stages of neural tube formation cells incorporate 3 H-thymidine into their nuclei, whereas during embryonic development some cells stop incorporating precursors for DNA synthesis because they are no longer involved in active mitosis. Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Springer-Verlag, Berlin, pp. Five- to six- m m sections were cut with a steel blade. In contrast, cryostatic sectioning of biological material is done at low temperatures with no need of exposure to organic solvents. In spite of their proximity, the dermatome and myotome showed marked differences in cell proliferation.

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The origin of these structures is directly related to cell proliferation and differentiation.

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However, no incorporation of BrdU was observed in myotomic cells, which give rise to axial skeletal muscle. Immunohistochemical localization of cellular components depends on adequate tissue preservation with no destruction of antigenic sites, and on the acquisition of sufficiently thin sections that provide a good resolution of subcellular structures Kimberly and Slpecky, However, the long time required by these procedures and the need to use potentially dangerous and expensive materials have led to the development of non-radioactive methods. Proliferating cells that did not reach the S phase, including young glia cells and neurons, did not incorporate BrdU and showed no colorimetric reaction. One such non-radioactive approach is based on the use of 5-bromo-2'-deoxyuridine BrdU. In an immunohistochemical analysis done using an antibody against desmin and acetylcholinesterase activity, Kaehn et al. This thymidine analog is incorporated into DNA during the S phase of the cell cycle and is detected in histological sections using antibodies conjugated to enzymes or fluorescent dyes Gratzner, ; Schutte et al.

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